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chip grade protein g magnetic beads  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc chip grade protein g magnetic beads
    Chip Grade Protein G Magnetic Beads, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 459 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chip+grade+protein+g+magnetic+beads/ChIP-Grade+Protein+G+Magnetic+Beads/pm42844247-434-0-5
    Average 96 stars, based on 459 article reviews
    chip grade protein g magnetic beads - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Chromatin Immunoprecipitation:

    Article Title: Transcriptional repression of TGFB2-AS1 by GATA6 drives triple-negative breast cancer metastasis
    Article Snippet: Keeping 10% of the sample as input DNA, the remaining samples with 10 μg GATA6 antibody (proteintech, #55435-1-AP), SMARCA4 antibody (CST, #12483T) or rabbit normal immunoglobulin (IgG) incubated overnight at 4 °C on a rotator. .. Next day, ChIP-Grade Protein G Magnetic Beads (CST, #9006) were used to precipitate the chromatin. ..

    Article Title: Mechanistic insights into CAR-mediated repression of the HNF4α-FABP1 axis and inhibition of HepG2 cell proliferation.
    Article Snippet: Twenty-four h after seeding, the cells were treated with Dox (1 μg/ml) or vehicle control for 72 h. These cells were then cross-linked by directly adding formaldehyde (final concentration, 1%) into medium, and incubated for 10 min at room temperature, washed with ice-cold phosphate-buffered saline containing a protease inhibitor cocktail (#7012, Cell Signaling Technology), lysed, and digested by Micrococcal Nuclease. .. Immunoprecipitation was performed overnight at 4◦C using anti-HNF4α (AB181604, Abcam), anti-AcetylHistone H3 (Lys9/Lys14) (#9677 s, Cell Signaling Technology) antibodies or normal IgG (#2729, Cell Signaling Technology) followed by precipitation using ChIP-Grade Protein G Magnetic Beads (#9006, Cell Signaling Technology). .. The immunoprecipitated DNA was purified according to the Cell Signaling ChIP Kit’s instruction.

    Article Title: Three alternative promoters govern PGC-1α expression in chicken and are directly repressed by PPARγ
    Article Snippet: The DNA fragments were then immunoprecipitated overnight at 4°C with an anti-PPARγ antibody (Cell Signaling Technology, #2443). .. Immune complexes were captured using ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology, USA), washed with low- and high-salt buffers, and subjected to crosslink reversal. ..

    Article Title: Histone H4K5 lactylation-driven feedback loop modulates glycerol-linked immunosuppressive activity of tumor-associated T regulatory cells.
    Article Snippet: .. ChIP-Grade Protein G Magnetic Beads (CST, #9006, with 3 μL per μg of antibody) were added and incubated at 4 °C for 2 hours. ..

    Article Title: NF‑κB‑driven LUZP1 promotes metastasis and chemoresistance in head and neck squamous cell carcinoma.
    Article Snippet: Immunoprecipitation with an anti‐histone h3 antibody (cat. no. 4620; Cell signaling Technology, Inc.) was included as a positive control. .. Immune complexes were captured using ChIP‐grade Protein g magnetic beads (cat. no. 9006; Cell signaling Technology, Inc.) and subjected to sequential low‐salt and high‐salt washes. .. Bound chromatin was eluted and reverse cross‐linked at 65 ̊C for 2 h in the presence of proteinase K, followed by DNA purification using spin columns and elution in DNA elution buffer. qPCR was performed using a Quantstudio 5 Real‐Time PCR system (Applied Biosystems, Thermo Fisher scientific, Inc.) with KAPA sYBR FAsT qPCR Master Mix (cat. no. KM4106; Roche Diagnostics). each reaction contained 1 μl of ChIP or input DNA in a total volume of 10 μl.

    Article Title: Transcriptional repression of TGFB2-AS1 by GATA6 drives triple-negative breast cancer metastasis.
    Article Snippet: Keeping 10% of the sample as input DNA, the remaining samples with 10 μg GATA6 antibody (proteintech, #55435- 1-AP), SMARCA4 antibody (CST, #12483T) or rabbit normal immunoglobulin (IgG) incubated overnight at 4 °C on a rotator. .. Next day, ChIP-Grade Protein G Magnetic Beads (CST, #9006) were used to precipitate the chromatin. ..

    Article Title: GPR3 is an immediate-early gene-like GPCR regulating CREB-dependent neuronal differentiation
    Article Snippet: Micrococcal Nuclease , Cell Signaling Technology , Cat# 10011. .. ChIP-Grade Protein G Magnetic Beads , Cell Signaling Technology , Cat# 9006. .. QIAquick PCR Purification Kit , Qiagen , Cat# 28104.

    Magnetic Beads:

    Article Title: Transcriptional repression of TGFB2-AS1 by GATA6 drives triple-negative breast cancer metastasis
    Article Snippet: Keeping 10% of the sample as input DNA, the remaining samples with 10 μg GATA6 antibody (proteintech, #55435-1-AP), SMARCA4 antibody (CST, #12483T) or rabbit normal immunoglobulin (IgG) incubated overnight at 4 °C on a rotator. .. Next day, ChIP-Grade Protein G Magnetic Beads (CST, #9006) were used to precipitate the chromatin. ..

    Article Title: Mechanistic insights into CAR-mediated repression of the HNF4α-FABP1 axis and inhibition of HepG2 cell proliferation.
    Article Snippet: Twenty-four h after seeding, the cells were treated with Dox (1 μg/ml) or vehicle control for 72 h. These cells were then cross-linked by directly adding formaldehyde (final concentration, 1%) into medium, and incubated for 10 min at room temperature, washed with ice-cold phosphate-buffered saline containing a protease inhibitor cocktail (#7012, Cell Signaling Technology), lysed, and digested by Micrococcal Nuclease. .. Immunoprecipitation was performed overnight at 4◦C using anti-HNF4α (AB181604, Abcam), anti-AcetylHistone H3 (Lys9/Lys14) (#9677 s, Cell Signaling Technology) antibodies or normal IgG (#2729, Cell Signaling Technology) followed by precipitation using ChIP-Grade Protein G Magnetic Beads (#9006, Cell Signaling Technology). .. The immunoprecipitated DNA was purified according to the Cell Signaling ChIP Kit’s instruction.

    Article Title: Three alternative promoters govern PGC-1α expression in chicken and are directly repressed by PPARγ
    Article Snippet: The DNA fragments were then immunoprecipitated overnight at 4°C with an anti-PPARγ antibody (Cell Signaling Technology, #2443). .. Immune complexes were captured using ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology, USA), washed with low- and high-salt buffers, and subjected to crosslink reversal. ..


    Article Title: NF‑κB‑driven LUZP1 promotes metastasis and chemoresistance in head and neck squamous cell carcinoma.
    Article Snippet: Immunoprecipitation with an anti‐histone h3 antibody (cat. no. 4620; Cell signaling Technology, Inc.) was included as a positive control. .. Immune complexes were captured using ChIP‐grade Protein g magnetic beads (cat. no. 9006; Cell signaling Technology, Inc.) and subjected to sequential low‐salt and high‐salt washes. .. Bound chromatin was eluted and reverse cross‐linked at 65 ̊C for 2 h in the presence of proteinase K, followed by DNA purification using spin columns and elution in DNA elution buffer. qPCR was performed using a Quantstudio 5 Real‐Time PCR system (Applied Biosystems, Thermo Fisher scientific, Inc.) with KAPA sYBR FAsT qPCR Master Mix (cat. no. KM4106; Roche Diagnostics). each reaction contained 1 μl of ChIP or input DNA in a total volume of 10 μl.

    Article Title: Transcriptional repression of TGFB2-AS1 by GATA6 drives triple-negative breast cancer metastasis.
    Article Snippet: Keeping 10% of the sample as input DNA, the remaining samples with 10 μg GATA6 antibody (proteintech, #55435- 1-AP), SMARCA4 antibody (CST, #12483T) or rabbit normal immunoglobulin (IgG) incubated overnight at 4 °C on a rotator. .. Next day, ChIP-Grade Protein G Magnetic Beads (CST, #9006) were used to precipitate the chromatin. ..

    Article Title: GPR3 is an immediate-early gene-like GPCR regulating CREB-dependent neuronal differentiation
    Article Snippet: Micrococcal Nuclease , Cell Signaling Technology , Cat# 10011. .. ChIP-Grade Protein G Magnetic Beads , Cell Signaling Technology , Cat# 9006. .. QIAquick PCR Purification Kit , Qiagen , Cat# 28104.

    Immunoprecipitation:

    Article Title: Mechanistic insights into CAR-mediated repression of the HNF4α-FABP1 axis and inhibition of HepG2 cell proliferation.
    Article Snippet: Twenty-four h after seeding, the cells were treated with Dox (1 μg/ml) or vehicle control for 72 h. These cells were then cross-linked by directly adding formaldehyde (final concentration, 1%) into medium, and incubated for 10 min at room temperature, washed with ice-cold phosphate-buffered saline containing a protease inhibitor cocktail (#7012, Cell Signaling Technology), lysed, and digested by Micrococcal Nuclease. .. Immunoprecipitation was performed overnight at 4◦C using anti-HNF4α (AB181604, Abcam), anti-AcetylHistone H3 (Lys9/Lys14) (#9677 s, Cell Signaling Technology) antibodies or normal IgG (#2729, Cell Signaling Technology) followed by precipitation using ChIP-Grade Protein G Magnetic Beads (#9006, Cell Signaling Technology). .. The immunoprecipitated DNA was purified according to the Cell Signaling ChIP Kit’s instruction.

    other:

    Article Title: GPR3 is an immediate-early gene-like GPCR regulating CREB-dependent neuronal differentiation
    Article Snippet: Chromatin immunoprecipitation assays were performed using the SimpleChIP Plus Enzymatic Chromatin IP Kit (Magnetic Beads) (Cell Signaling Technology, #9005) according to the manufacturer’s instructions.

    Cell-Signaling:

    Article Title: Three alternative promoters govern PGC-1α expression in chicken and are directly repressed by PPARγ
    Article Snippet: The DNA fragments were then immunoprecipitated overnight at 4°C with an anti-PPARγ antibody (Cell Signaling Technology, #2443). .. Immune complexes were captured using ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology, USA), washed with low- and high-salt buffers, and subjected to crosslink reversal. ..

    Article Title: GPR3 is an immediate-early gene-like GPCR regulating CREB-dependent neuronal differentiation
    Article Snippet: Micrococcal Nuclease , Cell Signaling Technology , Cat# 10011. .. ChIP-Grade Protein G Magnetic Beads , Cell Signaling Technology , Cat# 9006. .. QIAquick PCR Purification Kit , Qiagen , Cat# 28104.

    Incubation:




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